Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.

  title={Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.},
  author={Vincent J. Cannistraro and George D. Glekas and Christopher V. Rao and George W. Ordal},
  journal={Journal of bacteriology},
  volume={193 13},
The chemoreceptor-CheA kinase-CheW coupling protein complex, with ancillary associated proteins, is at the heart of chemotactic signal transduction in bacteria. The goal of this work was to determine the cellular stoichiometry of the chemotaxis signaling proteins in Bacillus subtilis. Quantitative immunoblotting was used to determine the total number of chemotaxis proteins in a single cell of B. subtilis. Significantly higher levels of chemoreceptors and much lower levels of CheA kinase were… CONTINUE READING

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