Cellular response of E. coli upon Hg2+ exposure--a case study of advanced nuclear analytical approach to metalloproteomics.

@article{Gao2013CellularRO,
  title={Cellular response of E. coli upon Hg2+ exposure--a case study of advanced nuclear analytical approach to metalloproteomics.},
  author={Yuxi Gao and Xiaomin Peng and Jinchao Zhang and Jiating Zhao and Yunyun Li and Yufeng Li and Bai Li and Yi Hu and Zhifang Chai},
  journal={Metallomics : integrated biometal science},
  year={2013},
  volume={5 7},
  pages={
          913-9
        }
}
The response of E. coli to Hg(2+) exposure was investigated using proteomic and metalloproteomic approaches. E. coli was cultured in the LB medium containing HgCl2 and/or selenomethionine. The growth curve of E. coli was measured to estimate the toxicity of Hg(2+) or selenomethionine. After two-dimensional gel electrophoresis (2-DE), distribution of Hg in 2-DE gel was detected with synchrotron radiation X-ray fluorescence (SRXRF) at 4W1B, Beijing Synchrotron Radiation Facility. The proteins… CONTINUE READING