Cellular response of E. coli upon Hg2+ exposure--a case study of advanced nuclear analytical approach to metalloproteomics.

  title={Cellular response of E. coli upon Hg2+ exposure--a case study of advanced nuclear analytical approach to metalloproteomics.},
  author={Yuxi Gao and Xiaomin Peng and J. Zhang and Jiating Zhao and Yunyun Li and Y. Li and Bai Li and Y. Hu and Zhifang Chai},
  journal={Metallomics : integrated biometal science},
  volume={5 7},
  • Yuxi Gao, Xiaomin Peng, +6 authors Zhifang Chai
  • Published 2013
  • Chemistry, Medicine
  • Metallomics : integrated biometal science
  • The response of E. coli to Hg(2+) exposure was investigated using proteomic and metalloproteomic approaches. E. coli was cultured in the LB medium containing HgCl2 and/or selenomethionine. The growth curve of E. coli was measured to estimate the toxicity of Hg(2+) or selenomethionine. After two-dimensional gel electrophoresis (2-DE), distribution of Hg in 2-DE gel was detected with synchrotron radiation X-ray fluorescence (SRXRF) at 4W1B, Beijing Synchrotron Radiation Facility. The proteins… CONTINUE READING
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