Cellular pathway(s) of antigen processing in fish APC: effect of varying in vitro temperatures on antigen catabolism.

@article{Vallejo1992CellularPO,
  title={Cellular pathway(s) of antigen processing in fish APC: effect of varying in vitro temperatures on antigen catabolism.},
  author={A N Vallejo and Norman W. Miller and L. William Clem},
  journal={Developmental and comparative immunology},
  year={1992},
  volume={16 5},
  pages={367-81}
}
Studies were conducted to determine the effects of varying in vitro temperatures during cellular processing of T-dependent antigens by catfish antigen-presenting cells (APC). Strategy involved pulsing of long-term monocyte lines (used as APC) with antigen at different temperatures for various periods of time and then fixing and coculturing with autologous peripheral blood leukocytes (PBL) as responders at a permissive temperature (i.e., 27 degrees C). Results showed that APC incubated with… CONTINUE READING

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