Cellular location of heat-labile enterotoxin in Escherichia coli.

@article{Hirst1984CellularLO,
  title={Cellular location of heat-labile enterotoxin in Escherichia coli.},
  author={Timothy R. Hirst and Linda L Randall and Simon J. S. Hardy},
  journal={Journal of bacteriology},
  year={1984},
  volume={157 2},
  pages={637-42}
}
We demonstrated that both the A and B subunits of heat-labile enterotoxin from Escherichia coli are located in the periplasm. The toxin was shown to form aggregates in Tris-EDTA buffers which are routinely used for isolating membranes. The aggregates pellet upon centrifugation, and this may explain why several previous investigators have concluded that enterotoxin is associated with membranes. 

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