Cellular distribution of phosphorothioate oligodeoxynucleotides in normal rodent tissues.

@article{Butler1997CellularDO,
  title={Cellular distribution of phosphorothioate oligodeoxynucleotides in normal rodent tissues.},
  author={Madeline Butler and Kimberly Stecker and Christopher F Bennett},
  journal={Laboratory investigation; a journal of technical methods and pathology},
  year={1997},
  volume={77 4},
  pages={379-88}
}
The distribution of intravenously injected phosphorothioate oligodeoxynucleotides (P = S ODN) was studied in vivo in rodent tissues using three histologic methods: immunohistochemistry with a monoclonal antibody that recognizes P = S ODN ISIS 2105; direct fluorescence microscopy of P = S ODN ISIS 2105 conjugated to rhodamine; and autoradiography of 14C-labeled P = S ODN ISIS 2302. All three methods gave the same pattern of oligonucleotide distribution, and the intensity of the histologic signal… CONTINUE READING