Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish

@inproceedings{Asakawa2013CellularDO,
  title={Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish},
  author={Kazuhide Asakawa and Gembu Abe and Koichi Kawakami},
  booktitle={Front. Neural Circuits},
  year={2013}
}
Bacterial artificial chromosome (BAC) transgenesis and gene/enhancer trapping are effective approaches for identification of genetically defined neuronal populations in the central nervous system (CNS). Here, we applied these techniques to zebrafish (Danio rerio) in order to obtain insights into the cellular architecture of the axial motor column in vertebrates. First, by using the BAC for the Mnx class homeodomain protein gene mnr2b/mnx2b, we established the mnGFF7 transgenic line expressing… CONTINUE READING