Cellular characterization of epidermal growth factor-expanded free-floating neurospheres.

  title={Cellular characterization of epidermal growth factor-expanded free-floating neurospheres.},
  author={Mar{\'i}a V T Lobo and Francisco Javier Alonso and Carolina Redondo and Miguel Angel L{\'o}pez-Toledano and Enrique Caso and Antonio S{\'a}nchez Herranz and Carlos Luis Pa{\'i}no and Diana Reimers and Eulalia Baz{\'a}n},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  volume={51 1},
Neural stem cells proliferate in liquid culture as cell clusters (neurospheres). This study was undertaken to characterize the epidermal growth factor (EGF)-expanded free-floating neurospheres derived from rat fetal striatum. We examined the ultrastructural and antigenic characteristics of these spheres. They consisted of two cell types, electron-dense and electron-lucent cells. Lucent cells were immunopositive to actin, vimentin, and nestin, whereas dense cells were immunopositive to actin… CONTINUE READING