Cellular attachment behavior on biodegradable polymer surface immobilizing endothelial cell-specific peptide

  title={Cellular attachment behavior on biodegradable polymer surface immobilizing endothelial cell-specific peptide},
  author={Yuichi Ohya and Kazuki Nishimura and Hiromichi Sumida and Yuta Yoshizaki and Akinori Kuzuya and Atsushi Mahara and Tetsuji Yamaoka},
  journal={Journal of Biomaterials Science, Polymer Edition},
  pages={1475 - 1488}
Abstract Small-caliber artificial blood vessels with inner diameters of smaller than 4 mm have not been put into practical use because of early thrombus formation and graft occlusion. To realize small-caliber artificial blood vessels with anti-thrombus property and long-term patency, one of the promising approaches is endothelialization of the lumen by tissue engineering approaches. Integrin α4β1 on the endothelial cell membrane is known to act as a receptor for Arg-Glu-Asp-Val (REDV) tetra… 
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A small-diameter synthetic vascular graft immobilized with the REDV peptide reduces early-stage fibrin clot deposition and results in graft patency in rats.
Results indicated that the REDV peptide on the luminal surface effectively reduced early stage fibrin clot deposition and formed the pseudo-endothelium layer in a peptide sequence-specific manner, resulting in graft patency.
Endothelialization strategy of implant materials surface: The newest research in recent 5 years.
This review summarizes the related research on the surface modification of materials by endothelialization in recent years, and analyzes the advantages and challenges of current endothelialized design ideas to find a more ideal surface modification strategy for future researchers to meet the requirements of clinical biocompatibility of cardiovascular materials.


Zwitterionic polycarboxybetaine coating functionalized with REDV peptide to improve selectivity for endothelial cells.
Experimental results proved that carboxybetaine-REDV coating maintained desirable antifouling ability and fine hemocompatibility, and was able to enhance the competitive growth of endothelial cells while limiting the adhesion, proliferation, and migration of smooth muscle cells.
Cell attachment and growth on films prepared from poly(depsipeptide-co-lactide) having various functional groups.
To evaluate the utility of these copolymers as functional scaffolds for tissue regeneration, the effects of reactive and ionic side-chain groups on cell attachment and growth were investigated using copolymer films with various numbers of carboxyl or amino groups.
Single-step immobilization of cell adhesive peptides on a variety of biomaterial substrates via tyrosine oxidation with copper catalyst and hydrogen peroxide.
A single-step immobilization of fibronectin-derived cell adhesive peptide (Arg-Glu-Asp-Val; REDV) onto polymer materials by use of tyrosine oxidation with copper catalyst and hydrogen peroxide is reported, resulting in enhanced adhesion of human umbilical vein endothelial cells.
Polymer Scaffolds for Small‐Diameter Vascular Tissue Engineering
To better engineer small-diameter blood vessels, a few types of novel scaffolds were fabricated from biodegradable poly(L-lactic acid) (PLLA) by means of thermally induced phase separation (TIPS) techniques by utilizing their advantageous structural features to facilitate blood vessel regeneration.