Cellular and microvascular changes of the ovarian follicle during folliculogenesis: a scanning electron microscopic study.

@article{Macchiarelli1992CellularAM,
  title={Cellular and microvascular changes of the ovarian follicle during folliculogenesis: a scanning electron microscopic study.},
  author={Guido Macchiarelli and E. Vizza and Stefania Annarita Nottola and Giuseppe Familiari and P. Motta},
  journal={Archives of histology and cytology},
  year={1992},
  volume={55 Suppl},
  pages={191-204}
}
In order to study the three-dimensional topographic arrangement of the oocyte and zona pellucida, follicular cells and follicle microvasculature, this study applied alkali maceration methods for tissue exposure, the ruthenium red-detergent method for the extracellular matrix visualization, and the vascular corrosion cast technique to rabbits, guinea-pigs and mice ovaries at different stages of follicular development. Macerated samples showed a gradual differentiation of the oocyte surface. This… CONTINUE READING

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