Cellular LanthaScreen and beta-lactamase reporter assays for high-throughput screening of JAK2 inhibitors.

@article{Robers2008CellularLA,
  title={Cellular LanthaScreen and beta-lactamase reporter assays for high-throughput screening of JAK2 inhibitors.},
  author={Matthew B. Robers and Thomas Machleidt and Coby B. Carlson and Kun Bi},
  journal={Assay and drug development technologies},
  year={2008},
  volume={6 4},
  pages={519-29}
}
The Janus kinase (JAK) 2/signal transducer and activator of transcription (STAT) 5 pathway is responsible for regulation of cellular responses to a number of cytokines and growth factors. In hematopoietic cells, growth factors such as granulocyte macrophage-colony stimulating factor, interleukin-3, and erythropoietin induce the activation of JAK2, which leads to the phosphorylation, dimerization, and transactivation of STAT5 proteins. Dysregulation of JAK2 by activating mutations such as… CONTINUE READING