Cells treated with TAP-2 antisense oligonucleotides are potent antigen-presenting cells in vitro and in vivo.

@article{Nair1996CellsTW,
  title={Cells treated with TAP-2 antisense oligonucleotides are potent antigen-presenting cells in vitro and in vivo.},
  author={Smita K Nair and David Snyder and Eli Gilboa},
  journal={Journal of immunology},
  year={1996},
  volume={156 5},
  pages={1772-80}
}
Treatment of RMA and EL4 cells or freshly isolated splenocytes with antisense (AS) oligonucleotides directed against the TAP-2 gene recreates the phenotype seen in cells that are genetically deficient in TAP function. Cells incubated with AS oligonucleotides exhibit reduced MHC class I expression on the cell surface, which can be increased by incubating the oligonucleotide-treated cells at 28 degrees C or by adding MHC haplotype-matched peptides to the culture medium. RMA cells or splenocytes… CONTINUE READING