Cells of proximal and distal tubular origin respond differently to challenges of oxalate and calcium oxalate crystals.

@article{Thamilselvan1999CellsOP,
  title={Cells of proximal and distal tubular origin respond differently to challenges of oxalate and calcium oxalate crystals.},
  author={Sivagnanam Thamilselvan and Raymond L. Hackett and Saeed R Khan},
  journal={Journal of the American Society of Nephrology : JASN},
  year={1999},
  volume={10 Suppl 14},
  pages={S452-6}
}
LLC-PK1 and Madin-Darby canine kidney (MDCK) cells were used to study the role of free radicals in renal epithelial injury during exposure to oxalate ions (Ox) and calcium oxalate monohydrate (COM) crystals. The cell cultures were exposed for 120 or 240 min to 1.0 mmol Ox or 1.0 mmol Ox plus 500 microg/ml of COM crystals averaging 1.0 microm in size. Exposure of both LLC-PK1 and MDCK cells to Ox alone increased the leakage of lactate dehydrogenase, which was further enhanced when cells were… CONTINUE READING