Cells deleted for Brca2 COOH terminus exhibit hypersensitivity to gamma-radiation and premature senescence.

@article{Morimatsu1998CellsDF,
  title={Cells deleted for Brca2 COOH terminus exhibit hypersensitivity to gamma-radiation and premature senescence.},
  author={Masami Morimatsu and Greg Donoho and Paul Hasty},
  journal={Cancer research},
  year={1998},
  volume={58 15},
  pages={3441-7}
}
The putative Brca2-MmRad51 interaction is analyzed in mouse cells deleted for the COOH terminus of Brca2 (amino acids 3140-3328), which contains a region that associates with MmRad51 by yeast two-hybrid. These cells are hypersensitive to gamma-radiation (suggesting defective recombinational repair) but not UV light (suggesting intact nucleotide excision repair) and maintain the G1-S and G2-M checkpoints after exposure to gamma-irradiation. Cells deleted for the COOH terminus of Brca2 progress… CONTINUE READING