Cell wall glycoproteins of Candida albicans as released by different methods.

@article{Casanova1991CellWG,
  title={Cell wall glycoproteins of Candida albicans as released by different methods.},
  author={Manuel Casanova and W. LaJean Chaffin},
  journal={Journal of general microbiology},
  year={1991},
  volume={137 5},
  pages={1045-51}
}
Different methods of extraction frequently used in other studies were used to release glycoproteins from both intact cells and isolated cell walls of yeast and hyphal forms of Candida albicans. Extracts were obtained from whole cells by treatment (i) with 2-mercaptoethanol (beta ME) at pH 8.6 and 37 C degrees and (ii) with zymolyase after treatment with beta ME. Extracts were obtained from isolated and washed cell walls (i) by boiling with beta ME and sodium dodecyl sulphate (SDS), (ii) by… CONTINUE READING
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