Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity

@article{RuizHerrera2003CellWC,
  title={Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity},
  author={Jos{\'e} Ruiz-Herrera and Patricia Garc{\'i}a-Maceira and Luis C. Castillo-Barahona and Eulogio Valent{\'i}n and R. Sentandreu},
  journal={Antonie van Leeuwenhoek},
  year={2003},
  volume={84},
  pages={229-238}
}
A collection of transposon-mutagenized strains of Yarrowia lipolytica was screened for wall defects by determination of their sensitivity to calcofluor white. A number of strains were hypersensitive, whereas others were resistant. Different non-allelic mutants displayed increased sensitivity to autolysis and lytic enzymes, independently of whether they were sensitive or resistant to calcofluor white. A thorough analysis of their cell walls revealed minor quantitative alterations, and no… CONTINUE READING
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