Cell-type-specific profiling of protein–DNA interactions without cell isolation using targeted DamID with next-generation sequencing

@article{Marshall2016CelltypespecificPO,
  title={Cell-type-specific profiling of protein–DNA interactions without cell isolation using targeted DamID with next-generation sequencing},
  author={Owen J. Marshall and Tony D Southall and Seth W. Cheetham and Andrea H. Brand},
  journal={Nature Protocols},
  year={2016},
  volume={11},
  pages={1586-1598}
}
This protocol is an extension to: Nat. Protoc. 2, 1467–1478 (2007); doi:10.1038/nprot.2007.148; published online 7 June 2007The ability to profile transcription and chromatin binding in a cell-type-specific manner is a powerful aid to understanding cell-fate specification and cellular function in multicellular organisms. We recently developed targeted DamID (TaDa) to enable genome-wide, cell-type-specific profiling of DNA- and chromatin-binding proteins in vivo without cell isolation. As a… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 18 CITATIONS

Developing DamID-seq to investigate transcription factor binding in mammalian cells

VIEW 5 EXCERPTS
CITES METHODS & BACKGROUND
HIGHLY INFLUENCED

References

Publications referenced by this paper.
SHOWING 1-10 OF 32 REFERENCES

Cell - type - specific profiling of gene expression and chromatin binding without cell isolation : assaying RNA Pol II occupancy in neural stem cells

T. D. Southall
  • Dev . Cell .
  • 2013

Cell type - specific chromatin immunoprecipitation from multicellular complex samples using BiTS - ChIP

S. Bonn
  • Nat . Protoc .
  • 2012