Cell-to-cell spread of HIV-1 occurs within minutes and may not involve the participation of virus particles.

@article{Sato1992CelltocellSO,
  title={Cell-to-cell spread of HIV-1 occurs within minutes and may not involve the participation of virus particles.},
  author={Hironori Sato and Jan Orenstein and Dimiter Dimitrov and Malcolm A Martin},
  journal={Virology},
  year={1992},
  volume={186 2},
  pages={712-24}
}
Although virus infections have been classically studied with "cell-free" virion preparations, many animal viruses are able to spread both in vitro and in vivo by inducing cell-cell fusion. An efficient system to monitor the cell-to-cell spread of HIV-1 has been developed employing chronically infected H9 donor cells. Under appropriate conditions of cocultivation with uninfected cells, the synthesis of unintegrated viral DNA, monitored by Southern blot hybridization, occurred between 2 and 4 hr… CONTINUE READING