Cell-surface expression of the channel activating protease xCAP-1 is required for activation of ENaC in the Xenopus oocyte.

@article{Vallet2002CellsurfaceEO,
  title={Cell-surface expression of the channel activating protease xCAP-1 is required for activation of ENaC in the Xenopus oocyte.},
  author={V{\'e}ronique Vallet and Corinne Pfister and Johannes Loffing and Bernard C Rossier},
  journal={Journal of the American Society of Nephrology : JASN},
  year={2002},
  volume={13 3},
  pages={588-94}
}
Sodium balance, extracellular fluid volume, and ultimately BP are maintained by precise regulation of the activity of the epithelial sodium channel (ENaC). Using a functional complementation assay in the Xenopus laevis oocyte expression system, a channel-activating protease (CAP-1) that increases ENaC activity two to threefold in the Xenopus oocyte expression system is here identified. External application of trypsin mimics the effect of Xenopus CAP-1 (xCAP-1) on ENaC activity, which can be… CONTINUE READING