Cell surface display system for Lactococcus lactis: a novel development for oral vaccine

  title={Cell surface display system for Lactococcus lactis: a novel development for oral vaccine},
  author={Abdul Rahim Raha and Nadimpalli R. S. Varma and Khatijah Yusoff and Ewan Ross and Hooi Ling Foo},
  journal={Applied Microbiology and Biotechnology},
The food-grade Lactococcus lactis is a potential vector to be used as a live vehicle for the delivery of heterologous proteins for vaccine and pharmaceutical purposes. We constructed a plasmid vector pSVac that harbors a 255-bp single-repeat sequence of the cell wall-binding protein region of the AcmA protein. The recombinant plasmid was transformed into Escherichia coli and expression of the gene fragment was driven by the T7 promoter of the plasmid. SDS-PAGE showed the presence of the… CONTINUE READING
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