Cell surface display of a β-glucosidase employing the type V secretion system on ethanologenic Escherichia coli for the fermentation of cellobiose to ethanol

@article{MuozGutirrez2012CellSD,
  title={Cell surface display of a β-glucosidase employing the type V secretion system on ethanologenic Escherichia coli for the fermentation of cellobiose to ethanol},
  author={Iv{\'a}n Mu{\~n}oz-Guti{\'e}rrez and Ricardo Oropeza and Guillermo Gosset and Alfredo Martinez},
  journal={Journal of Industrial Microbiology & Biotechnology},
  year={2012},
  volume={39},
  pages={1141-1152}
}
We used the autodisplay system AIDA-I, which belongs to the type V secretion system (TVSS), to display the β-glucosidase BglC from Thermobifida fusca on the outer membrane of the ethanologenic Escherichia coli strain MS04 (MG1655 ∆pflB, ∆adhE, ∆frdA, ∆xylFGH, ∆ldhA, PpflB::pdc Zm -adhB Zm ). MS04 that was transformed with the plasmid pAIDABglCRHis showed cellobiase activity (171 U/gCDW) and fermented 40 g/l cellobiose in mineral medium in 60 h with an ethanol yield of 81 % of the theoretical… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 15 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 38 references

Utilization of cellobiose by recombinant b-glucosidase-expressing strains of Saccharomyces cerevisiae: characterization and evaluation of the sufficiency of expression

JE McBride, JJ Zietsman, WH Van Zyl, LR Lynd
Enzyme Microb Technol • 2005
View 6 Excerpts
Highly Influenced

Advances in ethanol production.

Current opinion in biotechnology • 2011
View 6 Excerpts
Highly Influenced

Engineering Escherichia coli for efficient cellobiose utilization

Applied Microbiology and Biotechnology • 2011
View 3 Excerpts
Highly Influenced

Strains of Escherichia coli modified by metabolic engineering to produce chemical compounds from hydrolyzed lignocellulose

A Martı́nez Jiménez, G Gosset Lagarda, +3 authors J Utrilla Carreri
J Ind Microbiol Biotechnol • 2010
View 7 Excerpts
Highly Influenced

The autodisplay story, from discovery to biotechnical and biomedical applications.

Microbiology and molecular biology reviews : MMBR • 2007
View 6 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…