Cell surface binding of TIMP-2 and pro-MMP-2/TIMP-2 complex.

  title={Cell surface binding of TIMP-2 and pro-MMP-2/TIMP-2 complex.},
  author={Michael R. Emmert-Buck and Herv Emonard and M L Corcoran and Henry C. Krutzsch and Jean Michel Foidart and William G Stetler-Stevenson},
  journal={FEBS letters},
  volume={364 1},
Tissue inhibitor of metalloproteinases (TIMP-2) is a low molecular weight proteinase inhibitor capable of inhibiting activated matrix metalloproteinases (MMPs). TIMP-2 is found both free and in a 1:1 stoichiometric complex with the pro-enzyme form of MMP-2 (pro-MMP-2/TIMP-2 complex). We have measured the binding of recombinant TIMP-2 to intact HT-1080 and MCF-7 cells. HT-1080 cells in suspension bound 125I-labeled rTIMP-2 with a Kd of 2.5 nM and 30,000 sites/cell. Monolayers of MCF-7 cells were… CONTINUE READING


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