Cell signalling-mediating insulin increase of mRNA expression for cationic amino acid transporters-1 and -2 and membrane hyperpolarization in human umbilical vein endothelial cells

@article{Gonzlez2004CellSI,
  title={Cell signalling-mediating insulin increase of mRNA expression for cationic amino acid transporters-1 and -2 and membrane hyperpolarization in human umbilical vein endothelial cells},
  author={Marcelo Gonz{\'a}lez and Carlos Flores and Jeremy D. Pearson and Paola Casanello and Luis Sobrevia},
  journal={Pfl{\"u}gers Archiv},
  year={2004},
  volume={448},
  pages={383-394}
}
Insulin induces vasodilatation in human subjects and increases l-arginine transport and NO synthesis in human umbilical vein endothelial cells (HUVEC). Cell signalling events associated with insulin effects on activity and mRNA expression of the human cationic amino acid transporters 1 (hCAT-1) and 2B (hCAT-2B) are unknown. l-Arginine transport and eNOS activity were determined in HUVEC exposed to insulin. mRNA levels for hCAT-1, hCAT-2B and eNOS were quantitated by real time RT-PCR and… Expand
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TLDR
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