Cell separation on microfabricated electrodes using dielectrophoretic/gravitational field-flow fractionation.

@article{Yang1999CellSO,
  title={Cell separation on microfabricated electrodes using dielectrophoretic/gravitational field-flow fractionation.},
  author={Jin-Young Yang and Ying Huang and Xiaochun Wang and Frederick F. Becker and Peter R. C. Gascoyne},
  journal={Analytical chemistry},
  year={1999},
  volume={71 5},
  pages={911-8}
}
Dielectrophoretic/gravitational field-flow fractionation (DEP/G-FFF) was used to separate cultured human breast cancer MDA-435 cells from normal blood cells mixed together in a sucrose/dextrose medium. An array of microfabricated, interdigitated electrodes of 50 microns widths and spacings, and lining the bottom surface of a thin chamber (0.42 mm H x 25 mm W x 300 mm L), was used to generate DEP forces that levitated the cells. A 10-microL cell mixture sample containing approximately 50,000… CONTINUE READING