Cell patterning with mucin biopolymers.

@article{Crouzier2013CellPW,
  title={Cell patterning with mucin biopolymers.},
  author={Thomas Crouzier and Han Byul Jang and J Ahn and Roman Stocker and Katharina Ribbeck},
  journal={Biomacromolecules},
  year={2013},
  volume={14 9},
  pages={
          3010-6
        }
}
The precise spatial control of cell adhesion to surfaces is an endeavor that has enabled discoveries in cell biology and new possibilities in tissue engineering. The generation of cell-repellent surfaces currently requires advanced chemistry techniques and could be simplified. Here we show that mucins, glycoproteins of high structural and chemical complexity, spontaneously adsorb on hydrophobic substrates to form coatings that prevent the surface adhesion of mammalian epithelial cells… 

Figures from this paper

Modulating the Bioactivity of Mucin Hydrogels with Crosslinking Architecture
TLDR
It is shown that bovine submaxillary mucins, a highly glycosylated and immune‐modulating protein, exhibit strikingly different bioactivities whether they are crosslinked through their glycans or their protein domains.
Mucin-Inspired Lubrication on Hydrophobic Surfaces.
TLDR
It is demonstrated that the simple biopolysaccharide dextran can be functionalized with hydrophobic groups which confers efficient surface adsorption and good lubricity on PDMS to the poly Saccharide.
Mucins as multifunctional building blocks of biomaterials.
TLDR
A vision for how mucins could be used in the near future and what challenges await the field before biomaterials made of mucins and mucin-mimics can be translated into commercial products are offered.
Glyco-Modification of Mucin Hydrogels to Investigate Their Immune Activity
TLDR
The enzymatic glyco-modulation of Muc gels appears as a useful tool to help understand the biological functions of specific glycans on mucins which can further inform on their use in various biomedical applications.
Immune‐Modulating Mucin Hydrogel Microdroplets for the Encapsulation of Cell and Microtissue
TLDR
The combination of strong immune modulatory properties, appropriate selective barrier profile, biocompatibility for embedded cells Muc‐gels of particular value for microencapsulating cells or microtissues for transplantation.
Mucin hydrogel glyco-modulation to investigate immune activity of mucin glycans
TLDR
The enzymatic glyco-modulation of Muc gels appears as a useful tool to help understand the biological functions of specific glycans on mucins which can further inform on their use in various biomedical applications.
Micropatterned Cell‐Repellent Interface Using Femtosecond Laser Direct Writing to Engineer Controlled Cell Organization
TLDR
Using femtosecond‐laser direct writing to pattern surfaces with microcraters, it is shown that micropatterned surfaces with physical and chemical changes have outstanding characteristics of super‐hydrophobicity.
Comprehensive Evaluation of Stable Neuronal Cell Adhesion and Culture on One-Step Modified Polydimethylsiloxane Using Functionalized Pluronic
TLDR
A comprehensive evaluation of highly stable neuronal cell adhesion and culture on the PDMS surface modified in one step using functionalized Pluronic is presented, which is useful for potential applications in the fields of neurobiology, neuron microengineering, and brain-on-a-chip.
Covalent Mucin Coatings Form Stable Anti‐Biofouling Layers on a Broad Range of Medical Polymer Materials
TLDR
The coating mechanism described here introduces a promising strategy to overcome biofouling issues on a broad range of medical devices and exhibits excellent stability against mechanical, thermal, and chemical challenges.
...
...

References

SHOWING 1-10 OF 49 REFERENCES
Micropatterned Surfaces for Control of Cell Shape, Position, and Function
TLDR
Progressively restricting bovine and human endothelial cell extension by culturing cells on smaller and smaller micropatterned adhesive islands regulated a transition from growth to apoptosis on a single continuum of cell spreading, thus confirming the central role of cell shape in cell function.
Rapid multicomponent optical protein patterning.
TLDR
Improved laser-assisted protein adsorption by photobleaching (LAPAP) created arbitrary patterns made of several different proteins simultaneously, the fabrication time was reduced more than one order of magnitude and secondary antibodies were used to significantly enlarge the spectrum of proteins that can be employed.
Adhesion of microorganisms to bovine submaxillary mucin coatings: effect of coating deposition conditions
The adhesion of Staphylococcus epidermidis, Escherichia coli, and Candida albicans on mucin coatings was evaluated to explore the feasibility of using the coating to increase the infection resistance
Comparative study and improvement of current cell micro-patterning techniques.
TLDR
A new method based on UV crosslinking of adhesion proteins with benzophenone to easily and rapidly produce highly reproducible micropatterns without the use of a microfabricated elastomeric stamp is presented.
Sequential adsorption of bovine mucin and lactoperoxidase to various substrates studied with quartz crystal microbalance with dissipation.
TLDR
The quartz crystal microbalance with dissipation (QCM-D) technique has been used to extract information of the adsorption properties of bovine mucin (BSM) and lactoperoxidase (LPO) to gold, silica, and hydrophobized silica surfaces, and the interaction between the protein layers was interpreted as a combination of electrostatic andhydrophobic interactions.
Viscoelastic properties of fibrinogen adsorbed to the surface of biomaterials used in blood-contacting medical devices.
TLDR
This study addressed the question regarding to which extent systematic variations in polymer structure can be used to optimize X-ray visibility and provide tunable degradation rates while generating protein-repellant surface properties that minimize fibrinogen adsorption.
...
...