Cell passage-associated transient high oxygenation causes a transient decrease in cellular glutathione and affects T cell responses to apoptotic and mitogenic stimuli.

Abstract

Routine cell line maintenance involves removal of waste products and replenishment of nutrients via replacement of cell culture media. Here, we report that routine maintenance of three discrete cell lines (HSB-CCRF-2 and Jurkat T cells, and phaeo-chromocytoma PC12 cells) decreases the principal cellular antioxidant, glutathione, by up to 42% in HSB-CCRF-2… (More)
DOI: 10.1016/j.etap.2006.12.003

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@article{Grant2007CellPT, title={Cell passage-associated transient high oxygenation causes a transient decrease in cellular glutathione and affects T cell responses to apoptotic and mitogenic stimuli.}, author={Melissa M Grant and Helen R Griffiths}, journal={Environmental toxicology and pharmacology}, year={2007}, volume={23 3}, pages={335-9} }