Cell migration is essential for sustained growth of keratinocyte colonies: The roles of transforming growth factor-α and epidermal growth factor

@article{Barrandon1987CellMI,
  title={Cell migration is essential for sustained growth of keratinocyte colonies: The roles of transforming growth factor-$\alpha$ and epidermal growth factor},
  author={Yann Barrandon and Howard Green},
  journal={Cell},
  year={1987},
  volume={50},
  pages={1131-1137}
}
In common methods of cell cultivation, multiplication takes place in cells distributed uniformly or in small colonies and the number of cells increases exponentially. In contrast, an isolated colony of coherent epidermal keratinocytes, as it grows larger, departs drastically from exponential growth, and instead increases its radius at a constant rate over time. The rate of increase of colony radius is 8-fold greater in the presence of epidermal growth factor (EGF) and 10-fold greater in the… 
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References

SHOWING 1-10 OF 31 REFERENCES
Rapid induction of morphological changes in human carcinoma cells A-431 by epidermal growth factors
TLDR
Within the first hour of exposure to the hormone, the cell surface becomes exceedingly smooth and the nuclei seem to protrude above the plane of the otherwise thin monolayer, giving the cells a "fried egg" appearance.
Epidermal growth factor promotes the chemotactic migration of cultured rat intestinal epithelial cells
TLDR
It is concluded that EGF is a potent and specific chemo‐attractant for RIE‐1 intestinal epithelial cells and suggest that E GF might influence epithelial cell migration in vivo.
Growth factors from murine sarcoma virus-transformed cells.
  • J. D. De Larco, G. Todaro
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1978
TLDR
The sarcoma growth factors are a new class of polypeptide tropic factors that confer on fibroblasts in vitro properties associated with the transformed phenotype that are heat-stable, trypsin-sensitive, and active in nanogram quantities when tested for growth stimulation of untransformed rat and mouse fibro Blasts.
Three clonal types of keratinocyte with different capacities for multiplication.
  • Y. Barrandon, H. Green
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1987
TLDR
Colony-forming human epidermal cells are heterogeneous in their capacity for sustained growth and the incidence of the different clonal types is affected by aging, since cells originating from the epidermis of older donors give rise to a lower proportion of holoclones and a higher proportion of paraclones.
Sarcoma growth factor from conditioned medium of virally transformed cells is composed of both type alpha and type beta transforming growth factors.
TLDR
It is shown that further purification of crude SGF by reverse-phase HPLC on muBondapak C18 and CN columns at pH 2 resolves it into two distinctly different polypeptides, which are called types alpha and beta transforming growth factors (TGFs).
Cell size as a determinant of the clone-forming ability of human keratinocytes.
  • Y. Barrandon, H. Green
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1985
TLDR
It is concluded that when keratinocytes multiply rapidly, they extend reversibly the maximal size at which they are capable of generating clones into the range usually characteristic of terminally differentiating cells.
Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes
The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF seems to delay senescence
Seria cultivation of strains of human epidemal keratinocytes: the formation keratinizin colonies from single cell is
TLDR
Human diploid epidermis epidermal cells have been successfully grown in serial culture and it is possible to isolate keratinocyte clones free of viable fibroblasts, and human diploids keratinocytes appear to have a finite culture lifetime.
New evidence that growth in 3T3 cell cultures is a diffusion-limited process
TLDR
It is shown that the pattern of release from topoinhibition in a wounded culture can be distorted simply by inducing a gentle laminar flow of medium across the wound despite the reduced level of intercellular contact at wound edges.
Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin.
TLDR
Observations indicate that fibroblasts possess receptors for EGF by biological and physicochemical criteria, and suggest that a similar if not identical peptide may be amongst those factors in sera which stimulate cell growth.
...
1
2
3
4
...