Cell-mediated catabolism of aggrecan. Evidence that cleavage at the "aggrecanase" site (Glu373-Ala374) is a primary event in proteolysis of the interglobular domain.

@article{Lark1995CellmediatedCO,
  title={Cell-mediated catabolism of aggrecan. Evidence that cleavage at the "aggrecanase" site (Glu373-Ala374) is a primary event in proteolysis of the interglobular domain.},
  author={Michael W. Lark and J T Gordy and Jeffrey R. Weidner and J{\'e}sus Ayala and James H. Kimura and Hollis R. Williams and Richard A. Mumford and Carl R. Flannery and Steven S. Carlson and M Iwata},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 6},
  pages={2550-6}
}
A rat chondrosarcoma cell line and primary bovine chondrocytes have been used to study cell-mediated aggrecan catabolism. Addition of 1 microM retinoic acid to chondrosarcoma cultures resulted in aggrecan proteolysis with the release of greater than 90% of the cell layer aggrecan into the medium within 4 days. NH2-terminal sequencing of chondroitin sulfate-substituted catabolic products gave a single major NH2-terminal sequence of ARGNVILTXK, initiating at Ala374. This showed that the… CONTINUE READING

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