Cell lineage analysis by intracellular injection of fluorescent tracers.

@article{Weisblat1980CellLA,
  title={Cell lineage analysis by intracellular injection of fluorescent tracers.},
  author={David A. Weisblat and Saul L. Zackson and Seth S Blair and J. D. Young},
  journal={Science},
  year={1980},
  volume={209 4464},
  pages={
          1538-41
        }
}
Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast. 
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117 Citations
Background Citations
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Methods Citations
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Results Citations
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117 Citations

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Citation Type

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Dynamic lineage analysis of embryonic morphogenesis using transgenic quail and 4D multispectral imaging
We describe the development of transgenic quail that express various fluorescent proteins in targeted manners and their use as a model system that integrates advanced imaging approaches with…
Tissue-Specific Cell Lineages Originate in the Gastrula of the Zebrafish
TLDR
The divisions and migrations of individual cells were monitored during early development, revealing that in most cases the lineal descendants of single cells present at gastrula stage exclusively populate only single tissues, and may have stereotyped positional relationships within these tissues.
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References

SHOWING 1-6 OF 6 REFERENCES
Cell lineage analysis by intracellular injection of a tracer enzyme.
TLDR
Cell lineages during development of leeches can be ascertained by injection of horseradish peroxidase as a tracer into identified cells at early stages of embryogenesis, and the distribution of the tracer enzyme and hence of the progeny of the injected cell can be observed at a later stage of development by staining the preparation for horserothyrosine.
Embryonic cell lineages in the nervous system of the glossiphoniid leech Helobdella triserialis.
Embryonic development of the glossiphoniid leech Theromyzon rude: characterization of developmental stages.
Killing of single neurones by intracellular injection of proteolytic enzymes
TLDR
The central nervous system of the leech is used to establish a technique for destroying individual neurones one at a time and Pronase, a mixture of proteolytic enzymes, can be injected by pressure into an identified cell.
Size limit of molecules permeating the junctional membrane channels.
TLDR
The permeability of the cell-to-cell membrane channels in salivary gland cell junction (Chironomus thummi) was probed with fluorescent-labeled amino acids and synthetic or natural peptides to reflect the normal size limit for junctional channel permeation.
A direct approach to the structure of eukaryotic chromosomes.