Cell line‐specific control of recombinant monoclonal antibody production by CHO cells

@article{OCallaghan2010CellLC,
  title={Cell line‐specific control of recombinant monoclonal antibody production by CHO cells},
  author={Peter M. O'Callaghan and Jane McLeod and Leon P Pybus and Clare S Lovelady and Stephen J. Wilkinson and Andrew J Racher and Alison J. Porter and David C James},
  journal={Biotechnology and Bioengineering},
  year={2010},
  volume={106}
}
In this study we compare the cellular control of recombinant human IgG4 monoclonal antibody (Mab) synthesis in different CHO cell lines. Based on comprehensive empirical analyses of mRNA and polypeptide synthetic intermediates we constructed cell line‐specific mathematical models of recombinant Mab manufacture in seven GS‐CHO cell lines varying in specific production rate (qMab) over 350‐fold. This comparative analysis revealed that control of qMab involved both genetic construct and cell line… 
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TLDR
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Transcriptome dynamics of transgene amplification in Chinese hamster ovary cells
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It is hypothesize that the role of amplification is not merely increasing transcript levels, but also enriching survivors which have developed the cellular machinery for secreting proteins, leading to an increased frequency of isolating high‐producing clones.
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A novel role for HIF-1α in the age-associated metabolic changes that resulted in the phenotypic instability of a recombinant CHO cell line is suggested.
Culture temperature modulates aggregation of recombinant antibody in cho cells
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Observations indicate that low culture temperature can increase antibody aggregate formation in CHO cells by increasing LC and HC transcripts coupled with limited ER machinery.
Model‐directed engineering of “difficult‐to‐express” monoclonal antibody production by Chinese hamster ovary cells
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This study demonstrates that expression‐engineering strategies to improve production of DTE proteins in mammalian cells should be product specific, and based on rapid predictive tools to assess the relative impact of different engineering interventions.
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