Cell killing by lysosomotropic detergents

  title={Cell killing by lysosomotropic detergents},
  author={Douglas K. Miller and Elwyn Griffiths and John Lenard and Raymond A. Firestone},
  journal={The Journal of Cell Biology},
  pages={1841 - 1851}
We have studied the mechanism by which lysosomotropic detergents kill baby hamster kidney cells. Lysosomotropic detergents are lysosomotropic amines (compounds with pK between 5 and 9, such as imidazole or morpholine) containing straight-chain hydrocarbon "tails" of 9-14 carbon atoms (Firestone, R. A., J. M. Pisano, and R. J. Bonney. 1979, J. Med. Chem., 22:1130-1133). Using lucifer yellow CH as a specific fluorescent label for lysosomes, it was shown by light microscopy that N-dodecyl (C12… CONTINUE READING