Cell interactions with laminin and its proteolytic fragments during outgrowth of mouse primary trophoblast cells.

@article{Armant1991CellIW,
  title={Cell interactions with laminin and its proteolytic fragments during outgrowth of mouse primary trophoblast cells.},
  author={David Randall Armant},
  journal={Biology of reproduction},
  year={1991},
  volume={45 5},
  pages={
          664-72
        }
}
Mouse blastocysts in serum-free culture for 24-48 h become attachment-competent, adhere to fibronectin- or laminin-coated surfaces, and subsequently form trophoblast outgrowths. The blastocyst laminin receptor was characterized in outgrowth studies using modified laminin. Trophoblast cells interacted with the peptide portion of laminin, but not the oligosaccharide moiety since its adhesive activity was reduced by boiling or trypsin treatment, but not by treatments that removed or modified its… CONTINUE READING