Cell hybridization by electrofusion on filters.

@article{Ramos2002CellHB,
  title={Cell hybridization by electrofusion on filters.},
  author={Corinne Ramos and Deborah Bonenfant and Justin Teissi{\'e}},
  journal={Analytical biochemistry},
  year={2002},
  volume={302 2},
  pages={213-9}
}
Electric field pulses induce permeabilization and associated fusogenicity in cell membranes. Electrofusion of cells is usually performed in two steps: the first is the creation of close intercellular contacts; the second is an application of electric pulses that induces membrane fusion. Very large cell contacts can be obtained by a filter aspiration method. A cell monolayer is created by controlled suction on biocompatible filter. No spontaneous fusion results. Just after filtration… CONTINUE READING