Cell growth on immobilized cell growth factor. I. Acceleration of the growth of fibroblast cells on insulin-immobilized polymer matrix in culture medium without serum.

@article{Liu1992CellGO,
  title={Cell growth on immobilized cell growth factor. I. Acceleration of the growth of fibroblast cells on insulin-immobilized polymer matrix in culture medium without serum.},
  author={Sh. Q. Liu and Yosuke Ito and Yumi Imanishi},
  journal={Biomaterials},
  year={1992},
  volume={13 1},
  pages={50-8}
}
Culture of fibroblast cells in serum-free medium in the presence of insulin-immobilized films of poly(ethylene terephthalate) or poly(methyl methacrylate) accelerated the cell growth to several times that in the presence of free insulin molecules. The immobilization of insulin was useful also for elucidation of the mechanism of signal transmission through receptor molecules in the cell membrane. Free insulin molecules are known to be bound by receptors and the complex formed internalized and… CONTINUE READING