Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.

@article{Ueda2000CellgrowthCB,
  title={Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.},
  author={Hiroshi Ueda and Masahiro Kawahara and Takahide Aburatani and Kouhei Tsumoto and Kazuo Todokoro and Emi Suzuki and Hitoshi Nishimura and Paula A. Schueler and Gal Winter and Walter C. Mahoney and Izumi Kumagai and Teruyuki Nagamune},
  journal={Journal of immunological methods},
  year={2000},
  volume={241 1-2},
  pages={159-70}
}
Previously we have shown that the V(H) and V(L) fragments of an anti-hen egg lysozyme (HEL) antibody HyHEL-10 are weakly associated but can be driven together by antigen. By joining these antibody variable domains to the cytoplasmic portion of the murine erythropoietin receptor, we created a chimeric growth factor receptor that could be activated by HEL. After co-transfection with two plasmids encoding the respective chimeric receptors in IL-3 dependent murine pro-B Ba/F3 cells, a portion of… CONTINUE READING