Cell-free translation of carnation latent virus RNA and analysis of virus-specific dsRNA

@article{Meehan1991CellfreeTO,
  title={Cell-free translation of carnation latent virus RNA and analysis of virus-specific dsRNA},
  author={Brian M. Meehan and Peter R. Mills},
  journal={Virus Genes},
  year={1991},
  volume={5},
  pages={175-181}
}
Carnation latent virus was shown to direct the synthesis of virus-specific polypeptides in both reticulocyte lysate and wheat germ in vitro translation systems. The L-(4,5-3H)-leucine-labeled products ranged in molecular mass from Mr 190 to 33 kD. The 33 kD product, synthesized after only 15 min incubation, was the only major polypeptide that immunoprecipitated with antiserum to CarLV. Coatprotein synthesis does not occur as a result of proteolytic processing, but may arise as a result of… CONTINUE READING