Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis

@article{Kigawa1995CellfreeSA,
  title={Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis},
  author={Takanori Kigawa and Yutaka Muto and Shigeyuki Yokoyama},
  journal={Journal of Biomolecular NMR},
  year={1995},
  volume={6},
  pages={129-134}
}
SummaryFor the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity of labeling in in vivo expression systems. In the present study, a cell-free protein synthesis system was optimized, so that highly efficient and selective stable isotope labeling of proteins can be achieved in the absence of amino… Expand
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