Cell-free reconstitution of transport from the trans-golgi network to the late endosome/prevacuolar compartment.

@article{Blanchette2004CellfreeRO,
  title={Cell-free reconstitution of transport from the trans-golgi network to the late endosome/prevacuolar compartment.},
  author={Jennifer M. Blanchette and Mohamed E Abazeed and Robert S Fuller},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 47},
  pages={48767-73}
}
Vesicle-mediated transport between the trans-Golgi network (TGN) and the late endosome/prevacuolar compartment (PVC) is an essential step in lysosomal/vacuolar biogenesis. In addition, localization of integral membrane proteins to the TGN requires continual cycles of vesicular transport between the TGN and endosomal compartments. Genetic and biochemical analyses in yeast have identified a variety of proteins required for TGN-to-PVC transport. However, the precise mechanisms of vesicle formation… CONTINUE READING