Cell-free protein synthesis system prepared from insect cells by freeze-thawing.

  title={Cell-free protein synthesis system prepared from insect cells by freeze-thawing.},
  author={Toru Ezure and Takashi Suzuki and Shoken Higashide and Eiichi Shintani and Kohki Endo and Shin-ichiro Kobayashi and Masamitsu Shikata and Masaaki Ito and Koji Tanimizu and Osamu Nishimura},
  journal={Biotechnology progress},
  volume={22 6},
We established a novel cell-free protein synthesis system derived from Trichoplusia ni (HighFive) insect cells by a simple extraction method. Luciferase and beta-galactosidase were synthesized in this system with active forms. We analyzed and optimized (1) the preparation method of the insect cell extract, (2) the concentration of the reaction components, and (3) the 5'-untranslated region (5'-UTR) of mRNA. The extract was prepared by freeze-thawing insect cells suspended in the extraction… CONTINUE READING
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