Cell-free generation of the Notch1 intracellular domain (NICD) and APP-CTFγ

@article{Ikeuchi2002CellfreeGO,
  title={Cell-free generation of the Notch1 intracellular domain (NICD) and APP-CTF$\gamma$},
  author={Takeshi Ikeuchi and Sangram S. Sisodia},
  journal={NeuroMolecular Medicine},
  year={2002},
  volume={1},
  pages={43-54}
}
PSEN1 and PSEN2 encode polytopic membrane proteins, termed presenilin 1 (PS1) and presenilin 2 (PS2) that play an essential role in intramembranous (“γ-secretase”) proteolysis of selected type I membrane proteins, that include Notch1 and β-amyloid precursor protein (APP). In order to gain insights into biochemical mechanisms underlying γ-secretase processing of Notch1 and APP, we have developed a novel in vitro assay in which γ-secretase-mediated generation of S3/NICD and APP-CTFγ can be… Expand
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