Cell-free complements in vivo expression of the E. coli membrane proteome.

@article{Savage2007CellfreeCI,
  title={Cell-free complements in vivo expression of the E. coli membrane proteome.},
  author={David F Savage and Corey L. Anderson and Yaneth Robles-Colmenares and Zachary Eric Robert Newby and Robert Michael Stroud},
  journal={Protein science : a publication of the Protein Society},
  year={2007},
  volume={16 5},
  pages={
          966-76
        }
}
Reconstituted cell-free (CF) protein expression systems hold the promise of overcoming the traditional barriers associated with in vivo systems. This is particularly true for membrane proteins, which are often cytotoxic and due to the nature of the membrane, difficult to work with. To evaluate the potential of cell-free expression, we cloned 120 membrane proteins from E. coli and compared their expression profiles in both an E. coli in vivo system and an E. coli-derived cell-free system. Our… CONTINUE READING

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