Cell-free chemoenzymatic starch synthesis from carbon dioxide

  title={Cell-free chemoenzymatic starch synthesis from carbon dioxide},
  author={Tao Cai and Hongbing Sun and Jing Qiao and Leilei Zhu and Fan Zhang and J. Zhang and Zi Kang Tang and Xinlei Wei and Jiangang Yang and Qianqian Yuan and Wangyin Wang and Xue Yang and Huanyu Chu and Qian Wang and Chun You and Hongwu Ma and Yuanxia Sun and Yin Li and Can Li and Huifeng Jiang and Qinhong Wang and Yanhe Ma},
  pages={1523 - 1527}
Description From carbon dioxide to starch: no plants required Many plants turn glucose from photosynthesis into polymers that form insoluble starch granules ideal for long-term energy storage in roots and seeds. Cai et al. developed a hybrid system in which carbon dioxide is reduced to methanol by an inorganic catalyst and then converted by enzymes first to three and six carbon sugar units and then to polymeric starch. This artificial starch anabolic pathway relies on engineered recombinant… 
Upcycling CO2 into energy-rich long-chain compounds via electrochemical and metabolic engineering
A hybrid electro-biosystem, coupling spatially separate CO2 electrolysis with yeast fermentation, that efficiently converts CO2 to glucose with a high yield is described, illuminating the tantalizing possibility of a renewable-electricity-driven manufacturing industry.
A review of starch, a unique biopolymer - Structure, metabolism and in planta modifications.
Applications of Synthetic Biotechnology on Carbon Neutrality Research: A Review on Electrically Driven Microbial and Enzyme Engineering
The advances of the synthetic biotechnology method for the most promising organisms were reviewed, such as cyanobacteria, Escherichia coli, and yeast, in which the metabolic pathways were reconstructed to enhance the efficiency of CO2 capture and product synthesis.
Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients
This review highlights the recent development in metabolic engineering, the challenges in strain engineering and bioprocess design, and the perspectives of microbial utilization of C1 and C2 feedstocks for the biomanufacturing of value-added products.
Carbon dioxide photoreduction in prebiotic environments.
This work selected and collected papers related to photoreduction of carbon dioxide using compounds easily available on the Earth and considered of prebiotic relevance so that there could be a fruitful cross-fertilization of the two fields.
Engineering substrate specificity of HAD phosphatases and multienzyme systems development for the thermodynamic-driven manufacturing sugars
The structure-guided engineering of a phosphatase is reported, and variants with tailor-made preference for three widespread phosphorylated sugars are provided, namely, glucose 6-phosphate, fructose 6- phosphate, and mannose 6- phosphate, while simultaneously enhancement in catalytic efficiency is generated.
The integration of bio-catalysis and electrocatalysis to produce fuels and chemicals from carbon dioxide.
The dependence on fossil fuels has caused excessive emissions of greenhouse gases (GHGs), leading to climate changes and global warming. Even though the expansion of electricity generation will
Pyrolysis: A Convenient Route for Production of Eco-Friendly Fuels and Precursors for Chemical and Allied Industries
Thermochemical decomposition of post harvest agro-wastes (biomass) to solid carbonaceous material called as bio-char, condensable vapors (bio-oils and bio-tars) and non-condensable vapors (bio-gas or
Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation
Limiting proteolysis small molecule mapping (LiP-SMap) is a promising technique to explore and uncover novel post-translational metabolic regulation, although the method could benefit from improved sensitivity and specificity.


Enzymatic transformation of nonfood biomass to starch
A special polypeptide cap in potato alpha-glucan phosphorylase was essential to push a partially hydrolyzed intermediate of cellulose forward to the synthesis of amylose, paving the way for next-generation biorefineries based on simultaneous enzymatic biotransformation and microbial fermentation.
A synthetic pathway for the fixation of carbon dioxide in vitro
A synthetic cycle for the continuous fixation of CO2 in vitro using 17 enzymes from nine different organisms across all three domains of life, which is up to five times more efficient than the in vivo rates of the most common natural carbon fixation pathway.
Design and analysis of synthetic carbon fixation pathways
This study computationally identified alternative carbon fixation pathways that combine existing metabolic building blocks from various organisms using the entire repertoire of approximately 5,000 metabolic enzymes known to occur in nature, and suggests some of the proposed synthetic pathways could have significant quantitative advantages over their natural counterparts.
A synthetic biochemistry platform for cell free production of monoterpenes from glucose
A system comprises 27 enzymes for the conversion of glucose into monoterpenes that generates both NAD(P)H and ATP in a modified glucose breakdown module and utilizes both cofactors for building terpenes to highlight the potential of synthetic biochemistry approaches for producing bio-based chemicals.
Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design
The SACA pathway is proved to be the shortest, ATP-independent, carbon-conserving and oxygen-insensitive pathway for acetyl-CoA biosynthesis, opening possibilities for producing acetyl -CoA-derived chemicals from one-carbon resources in the future.
Isobutanol production freed from biological limits using synthetic biochemistry
A cell-free system in a bioreactor with continuous product removal that produces isobutanol from glucose at a maximum productivity, a titer of 275 g L−1 and 95% yield over the course of nearly 5 days is reported, suggesting that moving beyond cells has the potential to expand what is possible for bio-based chemical production.
Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts
This work uses microfluidics to develop a chloroplast mimic by encapsulating and operating photosynthetic membranes in cell-sized droplets that can be energized by light to power enzymes or enzyme cascades and analyzed for their catalytic properties in multiplex and real time.
A new-to-nature carboxylation module to improve natural and synthetic CO2 fixation
GCC is developed by combining rational design, high-throughput microfluidics and microplate screens, and its use for different applications is demonstrated, demonstrating how expanding the solution space of natural metabolism provides new opportunities for biotechnology and agriculture.