Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein.

@article{Roy2017CellfreeBF,
  title={Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein.},
  author={Shubhendu Sen Roy and Huy Xuan Nguyen and Thomas A Angelovich and Anna C. Hearps and Diana Huynh and Anthony Jaworowski and Theodoros Kelesidis},
  journal={Journal of visualized experiments : JoVE},
  year={2017},
  volume={128}
}
Low high-density lipoprotein cholesterol (HDL-C) levels are one of the most powerful independent negative predictors of atherosclerotic cardiovascular disease (CVD). The structure and function of HDL rather than HDL-C may more accurately predict atherosclerosis. Several HDL protein and lipid compositional changes that impair HDL function occur in inflammatory states such as atherosclerosis. HDL function is usually determined by cell based assays such as cholesterol efflux assay but these assays… CONTINUE READING