Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4.

@article{Hasbold1998CellDN,
  title={Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4.},
  author={Jhagvaral Hasbold and Alan Bruce Lyons and Marilyn R. Kehry and Philip D. Hodgkin},
  journal={European journal of immunology},
  year={1998},
  volume={28 3},
  pages={1040-51}
}
CD40 ligand (CD40L) and IL-4 are sufficient to induce resting murine B cells to divide and switch isotypes from IgM and IgD to IgG1 and IgE. Tracking of cell division following (5- and 6) carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling revealed that B cells expressed IgG1 after three cell divisions, and IgE after five. The probability of isotype switching at each division was independent of both time after stimulation and of the dose of CD40L. IL-4 concentration regulated the… CONTINUE READING