Cell-division control in Escherichia coli: specific induction of the SOS function SfiA protein is sufficient to block septation.

@article{Huisman1984CelldivisionCI,
  title={Cell-division control in Escherichia coli: specific induction of the SOS function SfiA protein is sufficient to block septation.},
  author={Olivier Huisman and R D'ari and Susan Gottesman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 14},
  pages={4490-4}
}
Blocks in DNA replication cause a rapid arrest of cell division in Escherichia coli. We have previously established that the function SfiA (SulA), induced under these conditions as part of the SOS response, is involved in this inhibition of division. To separate the effects of SfiA from those of other SOS functions, we have constructed a plac-sfiA operon fusion, permitting specific induction of SfiA protein by addition of the lac operon inducer isopropyl beta-D-thiogalactopyranoside (IPTG). In… CONTINUE READING