Cell dissociation with papain reduces the density of cGMP-activated channels of the retinal rod.

@article{Shen1995CellDW,
  title={Cell dissociation with papain reduces the density of cGMP-activated channels of the retinal rod.},
  author={J Shen and S Watanabe and Akimichi Kaneko},
  journal={The Japanese journal of physiology},
  year={1995},
  volume={45 1},
  pages={151-64}
}
A proteolytic enzyme, papain, is the enzyme commonly used for dissociation of retinal neurons. To ask whether papain modifies the activity of ion channels, cGMP-activated channels were recorded in inside-out membrane patches excised from the bullfrog rod outer segment, and the activity was compared between cells dissociated using papain and cells… CONTINUE READING