Cell cycle synchronization of tobacco BY-2 cells

  title={Cell cycle synchronization of tobacco BY-2 cells},
  author={Fumi Kumagai-Sano and Tomomi Hayashi and Toshio Sano and Seiichiro Hasezawa},
  journal={Nature Protocols},
Synchronization is a powerful technique for understanding cell cycle events. Here, we describe the procedure for synchronizing tobacco bright yellow 2 (BY-2) cell line, with which an exceptionally high level of synchrony can be achieved. It basically relies on an “arrest-and-release” strategy using aphidicolin, an inhibitor of DNA replication, and propyzamide, a plant-microtubule disruptant. In a single-step process using aphidicolin alone, a cell population with about 70% of the cells at… CONTINUE READING
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