Cell cycle specificity of tumor necrosis factor and its receptor.


Phase specificity in the TNF cytotoxic effect and the number of TNF binding receptors was investigated using L-M cells incubated synchronously from the S phase. TNF cytotoxicity was observed to occur at various levels during the cell cycle, with peak effect in the G2-M phase. Analysis with 125I-labeled TNF to determine the number of receptors binding TNF in the various cell phases shewed a phase specificity with the maximum number occurring in the G2-M phase, similar to the peak in cytotoxicity. The results suggest the existence of a cell cycle specificity in the cytotoxicity of TNF which is apparently related to changes in the number of receptors capable of binding TNF.


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@article{Watanabe1987CellCS, title={Cell cycle specificity of tumor necrosis factor and its receptor.}, author={Naoto Watanabe and Yoshiro Niitsu and Naoko Yamauchi and Hiroshi Neda and Hirohito Sone and M Maeda and Ichiro Urushizaki}, journal={Cell biology international reports}, year={1987}, volume={11 11}, pages={813-7} }