Cell-cycle-specific cDNAs from mammalian cells temperature sensitive for growth.

  title={Cell-cycle-specific cDNAs from mammalian cells temperature sensitive for growth.},
  author={R. R. Hirschhorn and Patricio Aller and Z. A. Yuan and Carolyn Winters Gibson and Renato Baserga},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={81 19},
A library of double-stranded cDNA was constructed from ts13 cells, a G1-specific temperature-sensitive hamster cell line. The cDNAs, cloned into pBR322, were prepared from poly(A)+ mRNA isolated from ts13 cells 6 hr after serum stimulation at the permissive temperature of 34 degrees C. Differential screening of the library with G1-specific and G0-specific single-stranded cDNA probes prepared from the same cells identified five cDNA clones whose sequences were preferentially expressed in G1… CONTINUE READING