Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A.

@article{Mudryj1991CellCR,
  title={Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A.},
  author={Maria M Mudryj and S H Devoto and Scott W. Hiebert and Tim Hunter and Joshua Pines and J. R. Nevins},
  journal={Cell},
  year={1991},
  volume={65 7},
  pages={1243-53}
}
We have examined E2F binding activity in extracts of synchronized NIH 3T3 cells. During the G0 to G1 transition, there is a marked increase in the level of active E2F. Subsequently, there are changes in the nature of E2F-containing complexes. A G1-specific complex increases in abundance, disappears, and is then replaced by another complex as S phase begins. Analysis of extracts of thymidine-blocked cells confirms that the complexes are cell cycle regulated. We also show that the cyclin A… CONTINUE READING