Cell cycle regulation of cloned cytolytic T cells by T cell growth factor: analysis by flow microfluorometry.

@article{Skaly1982CellCR,
  title={Cell cycle regulation of cloned cytolytic T cells by T cell growth factor: analysis by flow microfluorometry.},
  author={Rafick Pierre S{\'e}kaly and Hugh R. N. Macdonald and P. Zaech and Markus Nabholz},
  journal={Journal of immunology},
  year={1982},
  volume={129 4},
  pages={
          1407-14
        }
}
In an attempt to better define the mode of action of TCGF we have investigated the effect of complete removal of TCGF on the cell cycle kinetics of a TCGF-dependent murine CTL cloned line (B6.1). Cells that had been cultured in the presence or absence of TCGF were stained with propidium iodide, a DNA binding dye, and the distribution of DNA content was analyzed by flow cytometry. Results indicated that the proportion of cells entering the S phase of the cell cycle started to decrease 6 hr after… CONTINUE READING

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